Alessio Nahmad, PhD, Tel Aviv University, Tel Aviv, Israel, describes the role and applications of CRISPR-Cas9-based genome editing in the development of CAR T-cell and T cell receptor (TCR) therapies. The aim is often to ablate specific genes that can reduce efficiency of the T cell product such the TCR genes. For example, ablating TCR genes can improve efficiency and reduce toxicity. In addition, ablating certain genes, such as PD-1, can reduce cellular exhaustion. This interview took place at the International Conference on Lymphocyte Engineering (ICLE) 2022.