Saskia Rösch, PhD, Miltenyi Biotec, Bergisch Gladbach, Germany, discusses strategies to isolate and identify chimeric antigen receptor (CAR) from patient samples for the purposes of quality control. Dr Rösch outlines a CAR detection agent that finds active CARs by detecting CD19, CD22, CD33, and B-cell maturation antigen (BCMA) in the product, as well as measure their transduction rate. Monitoring the efficacy of CAR T-cell therapies can additionally be carried out by assessing metabolome of CAR T-cells. This interview took place at the International Conference on Lymphocyte Engineering (ICLE) 2022.