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WSCS 2021 | INCITE: a novel optical clearing technology for 3D imaging

Frank Marini, PhD, Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, provides an overview of index-matched clear imaging technology (INCITE). Animal tissues are made up of multiple components and the different refractive indices of each component makes tissues hard to view under a microscope. INCITE is optical clearing technology that utilizes propriety chemistry to fix animal tissues in a three-dimensional space, which is then run through a pressurized system to optically clear the tissue, making it transparent, thus homogenizing the refractive indices in the tissue. This technology offers the potential to improve the understanding of cellular interactions and spatial biology and could lead to the mapping of all cells in the human body to develop cell atlases. This interview took place at the 2021 World Stem Cell Summit (WSCS).