Frank Marini, PhD, Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, provides an overview of imaging techniques used in regenerative medicine. Improvements in optical imaging have resulted in an improved understanding of spatial biology and novel high throughput technologies with improved resolution such as multiphoton microscopy, lattice light-sheet microscopy and optical coherence tomography provide non-invasive imaging methods for a wide range of biological processes, including tumor formation or the effects of chemotherapy at a cellular level. Improving our knowledge of cellular biology in a three-dimensional space will be necessary in making advances within regenerative medicine. This interview took place at the 2021 World Stem Cell Summit (WSCS).