Bruce Levine, PhD, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, discusses advances in chimeric antigen receptor (CAR) T-cell therapy manufacturing, including developments in automation and shortened T-cell culture. Whilst a 24 hour manufacturing process has been in development, further research is required to assess feasibility. Dr Levine also describes T-Charge, a less labor-intensive novel CAR T-cell platform that expedites the culture time, requiring fewer reagents as a result. This interview took place at Advanced Therapies Week 2022.